Evaluation of copper resistant bacteria from vineyard ...
· Enrichment and Isolation of CopperResistant Bacteria. Enrichment of copper resistant bacteria was in 100 mL of nutrient broth (NB) (5 g of Peptone and 3 g of Beef extract) in 250 mL Erlenmeyer flasks to which 300 mg L −1 of Cu(II) as copper sulfate (CuSO 2 O) was added and pH was adjusted to NB was sterilized by autoclaving at 121°C for 20 min.